Reversed-Phase Liquid Chromatographic Method for Estrogen Determination in Equine Biological Samples
نویسندگان
چکیده
منابع مشابه
Reversed-phase liquid chromatographic method for estrogen determination in equine biological samples.
Equine unsaturated estrogens are the main components of brand formulations indicated for hormonal replacement therapy in both hypogonadic and postmenopausal women. These hormones are produced by the fetoplacental unit during equine gestation. A method is described for the quantitative determination of equilenin (EL), equilin (EQ), 17alpha-dihydroequilin (17dEQ), and estrone (El) in the plasma o...
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Easily degradating and various isomeric forms of rapamycin (Sirolimus) face the determination of this compound to many challenges. In this study, we developed and validated the isocratic reversed phase high performance liquid chromatographic (RP-HPLC) method for rapamycin. Separation was performed on a C8 column (MZ, 15 × 4.6 mm, 5 µm particle size) using methanol:water (80:20 v/v) as the mobil...
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easily degradating and various isomeric forms of rapamycin (sirolimus) face the determination of this compound to many challenges. in this study, we developed and validated the isocratic reversed phase high performance liquid chromatographic (rp-hplc) method for rapamycin. separation was performed on a c8 column (mz, 15 × 4.6 mm, 5 µm particle size) using methanol:water (80:20 v/v) as the mobil...
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A method for determination of riboflavin in animal feeds using liquid chromatography (LC) was developed for feed samples fortified with riboflavin at 1 mg/lb or greater (up to 10,000 mg/lb). Feed samples were extracted in 0.1 N HCl with heating on a steam bath for 30 min, followed immediately by mechanical shaking for 30 min. Sample extracts were diluted to target volume with 2% acetic acid and...
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ژورنال
عنوان ژورنال: Journal of Chromatographic Science
سال: 2001
ISSN: 0021-9665,1945-239X
DOI: 10.1093/chromsci/39.9.385